Effect of leukoreduction and temperature on risk of bacterial growth in CPDA ‐1 whole blood: A study of Escherichia coli

Background Collection of non-leukoreduced citrate-phosphate-dextrose-adenine (CPDA-1) whole blood is performed in walking banks. Blood collected under field conditions may have increased risk bacterial contamination. This study was conducted to examine the effects WBC reduction and storage temperature on growth Escherichia coli (ATCC® 25922™) CPDA-1 blood. Methods 450 ml from 10 group O donors inoculated with E. coli. Two hours after inoculation, test bags were leukoreduced a platelet-sparing filter. The control remained unfiltered. Each bag then split into three smaller for further at 2–6°C, 20–24°C, or 33–37°C. Bacterial quantified immediately, 2 3 h days 1, 3, 7, 14 all temperatures, 21 35 2–6°C. Results Whole median 19.5 (range 12.0–32.0) colony-forming units per (CFU/ml) After leukoreduction, 3.3 CFU/ml 0.0–33.3) remained. In arm, WBCs phagocytized within 24 20–24°C 33–37°C 9 bags. During slow self-sterilization occurred over time without leukoreduction. Conclusions Storage up before leukoreduction reduces coli-contamination Subsequent 2–6°C will reduce